Volume 7, Issue 3 (9-2019)
Jorjani Biomed J 2019, 7(3): 4-10 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Khosravi A, Kokabi F, Behzadi R, Asadi J. Tumorigenicity of Esophageal Cancer Stem Cells (ECSCs) in nude mouse xenograft model. Jorjani Biomed J 2019; 7 (3) :4-10
URL: http://goums.ac.ir/jorjanijournal/article-1-665-en.html
URL: http://goums.ac.ir/jorjanijournal/article-1-665-en.html
1- 1 Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran2 Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran
2- Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran
3- North Research Centre, Pasteur Institute of Iran, Amol, Iran
4- Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran , dr.asadi@goums.ac.ir
2- Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran
3- North Research Centre, Pasteur Institute of Iran, Amol, Iran
4- Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran , dr.asadi@goums.ac.ir
Abstract: (4211 Views)
Background and objectives: Modeling cancer in vivo is a very important tool to investigate cancer pathogenesis and molecular mechanisms involved in cancer progression. Laboratory mice are the most common animal used for rebuilding human cancer in vivo. Cancer stem cells (CSCs) are the main reason of failure in cancer therapy because of tumor relapse and metastasis. Isolation of cancer stem cells helps us to study their function and behavior. In the current study we separate cancer stem-like cells using sphere formation assay then investigate their tumorigenicity in xenograft tumor model.
Methods: YM1 cancer cells were cultured in serum-free media (SFM) in low adherent culture dishes for enrichment of cancer stem cells. The resulting spheres containing cancer stem-like cells were dissociated into single cells and were injected into the dorsal flank of B6 nude mice.
Results: A few days after injection, subcutaneous tumors formed. The growth curves of the resulting tumors were plotted using their weekly recorded lengths. The tumors' volume and weight were measured. The size of resulting tumors was appropriate to the number of cells injected. Pathological analysis confirmed esophageal origin of the resulting tumors.
Conclusion: Using laboratory mice models is a practical modeling system that provides us investigation of human tumors pathogenesis in vivo.
Methods: YM1 cancer cells were cultured in serum-free media (SFM) in low adherent culture dishes for enrichment of cancer stem cells. The resulting spheres containing cancer stem-like cells were dissociated into single cells and were injected into the dorsal flank of B6 nude mice.
Results: A few days after injection, subcutaneous tumors formed. The growth curves of the resulting tumors were plotted using their weekly recorded lengths. The tumors' volume and weight were measured. The size of resulting tumors was appropriate to the number of cells injected. Pathological analysis confirmed esophageal origin of the resulting tumors.
Conclusion: Using laboratory mice models is a practical modeling system that provides us investigation of human tumors pathogenesis in vivo.
Type of Article: Original article |
Subject:
Basic Medical Sciences
Received: 2019/05/9 | Accepted: 2019/08/26 | Published: 2019/09/1
Received: 2019/05/9 | Accepted: 2019/08/26 | Published: 2019/09/1
References
1. NAULT JC, BIOULAC-SAGE P, ZUCMAN-ROSSI J. Reviews in basic and clinical gastroenterology and hepatology. Gastroenterology. 2013;144:888-902. [DOI] [Google Scholar]
2. Ingelfinger JR, Rustgi A, El-Serag H. Esophageal carcinoma. N Engl J Med. 2014;371:2499-509. [view at publisher] [DOI] [Google Scholar]
3. Kamangar F, Dores GM, Anderson WF. Patterns of cancer incidence, mortality, and prevalence across five continents: defining priorities to reduce cancer disparities in different geographic regions of the world. Journal of clinical oncology. 2006;24(14):2137-50. [view at publisher] [DOI] [Google Scholar]
4. Enzinger PC, Ilson DH, Kelsen DP. Chemotherapy in esophageal cancer. Seminars in oncology. 1999;26(5 Suppl 15):12-20. [Google Scholar]
5. Pennathur A, Farkas A, Krasinskas AM, Ferson PF, Gooding WE, Gibson MK, et al. Esophagectomy for T1 esophageal cancer: outcomes in 100 patients and implications for endoscopic therapy. The Annals of thoracic surgery. 2009;87(4):1048-55. [view at publisher] [DOI] [Google Scholar]
6. Shultz LD, Goodwin N, Ishikawa F, Hosur V, Lyons BL, Greiner DL. Human cancer growth and therapy in NOD/SCID/IL2Rγnull (NSG) mice. Cold Spring Harbor protocols. 2014;2014(7):694. [view at publisher] [DOI] [Google Scholar]
7. Saxena M, Christofori G. Rebuilding cancer metastasis in the mouse. Molecular oncology. 2013;7(2):283-96. [view at publisher] [DOI] [Google Scholar]
8. Berghmans S, Jette C, Langenau D, Hsu K, Stewart R, Look T, et al. Making waves in cancer research: new models in the zebrafish. Biotechniques. 2005;39(2):227-37. [DOI] [Google Scholar]
9. Khanna C, Hunter K. Modeling metastasis in vivo. Carcinogenesis. 2005;26(3):513-23. [DOI] [Google Scholar]
10. Blouin S, Baslé MF, Chappard D. Rat models of bone metastases. Clinical & experimental metastasis. 2005;22(8):605. [DOI] [Google Scholar]
11. Niu H-x, Du T, Xu Z-f, Zhang X-k, Wang R-g. Role of wild type p53 and double suicide genes in interventional therapy of liver cancer in rabbits. Acta cirurgica brasileira. 2012;27(8):522-8. [DOI] [Google Scholar]
12. Hubbard G, Wood D, Butcher W. Mammary carcinoma with metastasis in a rhesus monkey (Macaca mulatta). Veterinary pathology. 1984;21(5):531-3. [DOI] [Google Scholar]
13. Fantozzi A, Christofori G. Mouse models of breast cancer metastasis. Breast Cancer Research. 2006;8(4):212. [DOI] [Google Scholar]
14. Lapidot T, Sirard C, Vormoor J, Murdoch B, Hoang T, Caceres-Cortes J, et al. A cell initiating human acute myeloid leukaemia after transplantation into SCID mice. nature. 1994;367(6464):645. [DOI] [Google Scholar]
15. Al-Hajj M, Wicha MS, Benito-Hernandez A, Morrison SJ, Clarke MF. Prospective identification of tumorigenic breast cancer cells. Proceedings of the National Academy of Sciences. 2003;100(7):3983-8. [DOI] [Google Scholar]
16. Singh SK, Hawkins C, Clarke ID, Squire JA, Bayani J, Hide T, et al. Identification of human brain tumour initiating cells. nature. 2004;432(7015):396. [view at publisher] [DOI] [Google Scholar]
17. Dalerba P, Dylla SJ, Park I-K, Liu R, Wang X, Cho RW, et al. Phenotypic characterization of human colorectal cancer stem cells. Proceedings of the National Academy of Sciences. 2007;104(24):10158-63. [view at publisher] [DOI] [Google Scholar]
18. Zhang S, Balch C, Chan MW, Lai H-C, Matei D, Schilder JM, et al. Identification and characterization of ovarian cancer-initiating cells from primary human tumors. Cancer research. 2008;68(11):4311-20. [view at publisher] [DOI] [Google Scholar]
19. Hermann PC, Huber SL, Herrler T, Aicher A, Ellwart JW, Guba M, et al. Distinct populations of cancer stem cells determine tumor growth and metastatic activity in human pancreatic cancer. Cell stem cell. 2007;1(3):313-23. [DOI] [Google Scholar]
20. Shiozawa Y, Nie B, Pienta KJ, Morgan TM, Taichman RS. Cancer stem cells and their role in metastasis. Pharmacology & therapeutics. 2013;138(2):285-93. [DOI] [Google Scholar]
21. Pastrana E, Silva-Vargas V, Doetsch F. Eyes wide open: a critical review of sphere-formation as an assay for stem cells. Cell stem cell. 2011;8(5):486-98. [DOI] [Google Scholar]
22. Chen Y-C, Ingram PN, Fouladdel S, McDermott SP, Azizi E, Wicha MS, et al. High-throughput single-cell derived sphere formation for cancer stem-like cell identification and analysis. Scientific reports. 2016;6:27301. [view at publisher] [DOI] [Google Scholar]
23. Ayyoob K, Masoud K, Vahideh K, Jahanbakhsh A. Authentication of newly established human esophageal squamous cell carcinoma cell line (YM-1) using short tandem repeat (STR) profiling method. Tumor Biology. 2016;37(3):3197-204. [DOI] [Google Scholar]
24. Tomayko MM, Reynolds CP. Determination of subcutaneous tumor size in athymic (nude) mice. Cancer chemotherapy and pharmacology. 1989;24(3):148-54. [DOI] [Google Scholar]
25. Frese KK, Tuveson DA. Maximizing mouse cancer models. Nature Reviews Cancer. 2007;7(9):654. [view at publisher] [DOI] [Google Scholar]
26. Maddison K, Clarke AR. New approaches for modelling cancer mechanisms in the mouse. The Journal of Pathology: A Journal of the Pathological Society of Great Britain and Ireland. 2005;205(2):181-93. [view at publisher] [DOI] [Google Scholar]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
