Volume 7, Issue 2 (7-2019)                   Jorjani Biomed J 2019, 7(2): 61-70 | Back to browse issues page

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ajam F, Aghaei M, Mohammadi S, Saeedi M, Behnampour N, Memarian A. Increased expression of PD-1 on CD4+ T cells subsets in Rheumatoid Arthritis patients. Jorjani Biomed J. 2019; 7 (2) :61-70
URL: http://goums.ac.ir/jorjanijournal/article-1-664-en.html
1- Student Research Committee, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
2- Golestan Rheumatology Research Center (GRRC), Golestan University of Medical Sciences, Gorgan, Iran
3- Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran
4- Public Health Department, Faculty of Health, Golestan University of Medical Sciences, Gorgan, Iran
5- Golestan Rheumatology Research Center (GRRC), Golestan University of Medical Sciences, Gorgan, IranGolestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran , alimemarian@goums.ac.ir
Abstract:   (1842 Views)
Study objectives: Rheumatoid arthritis (RA) is a common chronic and systemic autoimmune disease, characterized by inflammation and the destruction of the joints. It is well known that CD4+ T cells play a major role in the pathogenesis of RA. Expanded subpopulations of CD4+ T cells have been reported in RA patients. Here, we investigated the expression of PD-1 on subsets of CD4+ T cells (CD4+CD28- and CD4+CD28+ T cells) in the peripheral blood (PB) and synovial fluid (SF) of patients with RA.
Methods: A total of 42 RA patients, including 10 newly diagnosed (ND) and 32 relapsed (RL) cases and also 20 healthy controls were enrolled. Phenotypic characterization subsets of CD4+ T cells were evaluated by flow cytometry, using fluorescence conjugated specific human monoclonal antibodies.
Results: The frequency of CD4+CD28+ T cells was significantly increased in SF versus PB in ND and RL patients. In contrast, the percentage of CD4+CD28- T cells was elevated in PB of ND and RL patients comparison to SF. Expression of PD-1 on CD4+CD28+ and CD4+CD28- T cells in PB of ND and RL patients was significantly higher than the healthy controls. Furthermore, PD-1 expression on CD4+CD28+ and CD4+CD28- T cells in SF versus PB of RL patients were significant increased.
Conclusion: These data suggest that CD4+ T cells subsets in RA patients were resistance to PD-1 mediated effects and PD-1 has insufficient ability to suppression of CD4+T cells.
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Type of Article: Original article | Subject: Basic Medical Sciences
Received: 2019/02/28 | Accepted: 2019/06/7 | Published: 2019/07/1

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