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Showing 3 results for Cerebrospinal Fluid

Moradi Av, Azadfar S, Fatemehcheraghali, Javid N, Ghaemi A, Tabarraei A,
Volume 5, Issue 2 (10-2011)
Abstract

Abstract Background and objectives: Mumps virus is one of the first known causative agents of meningitis in children. On-time diagnosis is the first step in treating meningitis. We aimed to evaluate Mumps virus meningitis in children in Gorgan, Iran Material and Methods: CSF and blood samples were taken from children with meningitis, Jun 2008 till Sep 2010. For 40 samples with negative bacterial culture, Extraction of viral RNA was carried out and Real-time PCR was performed for detection of Mumps virus. Demographic, clinical, biochemical and cytological data were collected. We run SPSS version 18 to analyze the data, using Chi Square (p<0.05). Results: three (7.5 %) samples have Mumps virus, two boys and one girl. All three positive cases have 0.5-1 degrees Celsius fever and vomiting but no bulging fontanel. They have not Kernig, Rodor, Brudzinski’s sign, hepatosplenomegaly, lymphadenopathy, pharyngitis and rash. ESR is higher than normal in all positive cases and CRP is positive in two cases. Protein of CSF in one case is higher than normal range. Conclusion: meningitis is an emergency condition therefore, molecular diagnostic techniques are recommended for early diagnosis and intervention. Key words: meningitis, mumps virus, cerebrospinal fluid, Real-Time PCR
Khoshdel Rad N, Mashayekhi F, Mirzajani E,
Volume 6, Issue 1 (4-2012)
Abstract

Abstract Background and objectives: C-Met is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor (HGFR). The HGF receptor possesses tyrosine -kinase activity and it is essential for embryonic development, wound healing and cancer. Many proteins are proteolytically released from the surface by a process known as ectodomain shedding. Shedding occurs under normal physiologic conditions and can be increased in certain pathologies. C-Met can be seen among many receptors for which ectodomain shedding has been shown. The aim of this study was to determine the concentration of soluble c-Met in the cerebrospinal fluid (CSF) and serum samples of patients with viral and bacterial meningitis. Material and Methods: in this study, 75 CSF and serum samples of patients with bacterial meningitis, 71 with viral meningitis and 82 normal controls were investigated. The soluble c-Met concentration was determined by enzyme linked immunosorbent assay (ELISA). Result: the amount of soluble c-met in CSF of patients with bacterial meningitis ( 83.91±5.50), viral meningitis ( 80.41±4.71) and control group ( 22.66±3.39) are compared with that in serum of patients with bacterial meningitis ( 561.58±25.87), viral meningitis ( 550.50 ±34.34) and control group ( 256.25±18.55). There is significant increase in the CSF and serum’s soluble c-Met expression in the patients with meningitis, in comparison with control group. Conclusion: The data presented here indicate that soluble c-Met is a constant component of human serum and CSF, but it can not be used for differentiating bacterial meningitis from viral meningitis. Key words: Soluble c-Met, concentration, cerebrospinal fluid, serum, meningitis
F Mashayekhi, F Rajaei,
Volume 6, Issue 2 (10-2012)
Abstract

Abstract Background and objectives: Meningitis is one of the most common infectious of the central nervous system (CNS), defined as an inflammation of the meninges. LIF is a potent pro-inflammatory factor. Cerebrospinal fluid (CSF) contains the growth factors and cytokines whose concentrations have been changed in most neurological diseases. The aim of this study was to determine the LIF concentration of serum and CSF in the children with bacterial meningitis. Material and Methods: In this study, the total protein concentration (TPC) and LIF in the serum and CSF of normal subjects and children with bacterial meningitis were measured by enzyme linked immunosorbent assay (ELISA). Results: the Values of serum TPC for children with meningitis (74.17±7.73 g/L) and controls (73.50±7.28 g/L) are not different significantly (P=0.7), and the TPC in the CSF of children suffering from meningitis and controls are 35±0.03 and 0.34±0.05 g/L, respectively (P=0.65). The concentration of serum LIF for children with meningitis( 253±19.14 ng/ml) is higher than that of controls (49.75±8.97 ng/ml), and also the concentration of LIF in the CSF of the children with meningitis (116.25±8.60 ng/ml ) is significantly higher than that of controls which is 9.04±1.83ng/ml (P<0.001). Conclusion: The LIF concentration in the CSF and serum may provide additional information in the differential diagnosis of meningitis. It is also concluded that LIF could be significantly involved in the pathophysiology of meningitis. Key words: Serum, Cerebrospinal fluid, Leukemia inhibitory factor, Children, Bacterial meningitis

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