Showing 9 results for Yazdani
N Keyhanvar, A Tabarraei, Y Yazdani,
Volume 7, Issue 2 (summer[PERSIAN] 2013)
Abstract
Abstract Background and objective: Hepcidin is a cystein-rich antimicrobial peptide, which is secreted by the liver. It fights against wide spectrum of bacteria, viruses and fungi and it is a major regulator of iron homeostasis. Today, scientists have made many efforts on the production of hepcidin. Baculovirus expression system is one of the best eukaryotic expression systems for production of recombinant hepcidin and production of the recombinant vector is one of the most important steps in this expression system. Material & Methods: First, the total RNA was separated from HepG2 cell line as a source of hepcidin expression. Then, after synthesis of total cDNA, human hepcidin sequence was amplified, using specific primers by PCR method. Next, hepcidin sequence was cloned into pTZ57R/T vector. After digestion of recombinant vector using ECoRI and BamHI restriction enzymes, recombinant pFastBac HT B vector containing human hepcidin cDNA was produced. Results: Coding sequence of human hepcidin is correctly cloned into pTZ57R/T vector and sub cloning into pFastBac HT B vector is performed successfully. The presence of a clear band near 274 bp resulted from PCR amplification and restriction enzyme are the confirmation of the cloning of human hepcidin. Conclusion: According to our knowledge, the present study is the first work that focuses on recombinant vector containing coding sequence of human prohepcidin. This recombinant vector can be used for human hepcidin production. Key words: Vector, Hepcidin, Iron
N Hashemi, Y Yazdani,
Volume 8, Issue 3 (Autumn[PERSIAN] 2014)
Abstract
Abstract
Background and Objectives: Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease. Mucosal feeding of myelin basic protein binding to the cholera toxin B subunit can reduce the intensity of the immune response in MS patients. Expression system, the domain composition of the fusion protein, accessibility of two domains, codon adaptation index (CAI) and GC contents are very important for the large scale production of fusion protein.
Material and Methods: we used DNA2, PSIPRED and ProtParam softwares for designing the best form to produce fusion protein. Moreover, the correct open reading frame of myelin basic protein was also considered. First the coding sequence was verified and then synthesized. For confirmation of the recombinant vector, PCR test was carried out using T7 primers. Finally it was inserted into the cloning site of pET28 expression vector.
Results: After coding optimization, the CAI rate was increased from 64 % to 80% and GC content from 41 % to 49%. The presence of a band near 700bp resulted from PCR amplification test demonstrates the correct cloning of recombinant vectors in the cloning site of pET28 expression vector.
Conclusion: According to software and experimental analysis, the designed sequence probably in the best form could be used for production of recombinant protein.
Keywords: Multiple Sclerosis, Cholera Toxin, Myelin Basic Protein
H Haghighatfard, Y Yazdani, Y.,
Volume 9, Issue 1 (March, April[PERSIAN] 2015)
Abstract
Abstract
Background and Objective: the inhibition of tumor-associated angiogenesis can significantly reduce the tumor proliferation. The basic fibroblast growth factor (bFGF), an important angiogenic factor, is considered as a potential therapeutic target for cancer therapy. The purpose of this study was evaluating, designing and construction of new recombinant DNA molecule in order to have efficient expression of a fusion protein consisting of the bFGF and immunodominant epitopes of Pseudomonas toxin.
Material and Methods: Different types of peptide linker, codon adaptation index (CAI) and adding signal peptide were considered in designing of immunogenic coding sequence. After software evaluation, the recombinant DNA molecule was ordered in the puc57 cloning vector. Then, coding sequence inserted into the multiple cloning site of pET28-a plasmid. Finally, PCR and enzymatic digestion tests were done for evaluation of recombinant expression vector.
Results: Optimization of DNA sequence, codon adaptation index (CAI) increased from 0.69 to 0.83 and GC content decreased from 61 to 54.77. The presence of 1214-bp PCR product and 1029-bp one obtaining from enzymatic digestion confirmed the correction of the cloning process.
Conclusion: According to the previous studies, it is the first work for designing, optimizing and synthesis of recombinant DNA consisting of bFGF and immunodominant epitopes of Pseudomonas toxin.
Keywords: Tumor angiogenesis, immunodominant epitopres of Pseudomonas toxin, Fibroblast growth factor 2, DNA 2 software
Mehdi Movaghari, Shaghayegh Anvari, Aylar Jamali, Mahin Yazdani,
Volume 11, Issue 3 (May-Jun 2017)
Abstract
ABSTRACT
Background and Objective: Vibrio is a genus of bacteria that are widely distributed in aquatic environments. The genus includes several important pathogens that endanger farm animals and humans who ingest seafood or water contaminated with the bacteria. Virulence of Vibrio spp. is regulated by ctxAB and tcpA genes. The aim of this study was to determine the prevalence of Vibrio spp., and tcpA and ctxB virulence genes in isolates from surface water and salt water samples collected from Golestan Province, Iran.
Methods: Overall, 115 water samples were collected from the Caspian Sea coast, lagoons and rivers in the Golestan Province. The samples were filtered by membrane filtration method, and enriched in alkaline peptone water with 1% NaCl. The isolates were grown on TCBS agar, and identified by biochemical tests. Presence of the tcpA and ctxB virulence genes was investigated by polymerase chain reaction.
Results: In this study, Vibrio alginolyticus was the predominant species (38%) isolated from the seawater and surface water samples, followed by Vibrio parahaemolyticus (23%), Vibrio harvei (15%), Vibrio fluvialis (14%) and Vibrio damsela (10%). The virulence genes were not detected in any of the isolates found in the study.
Conclusion: This study indicates that V. alginolyticus is the most prevalent Vibrio spp. in surface water and seawater samples collected from the Golestan Province, Iran.
Keywords: Environmental Vibrio, Surface water, ctx B gene, tcpA gene.
Hadi Bazzazi, Yaghoub Yazdani, Nasser Behnampour, Hadi Hossein-Nataj, Ali Memarian, Mehrdad Aghaei,
Volume 13, Issue 6 (Nov-Dec 2019)
Abstract
ABSTRACT
Background and Objectives: T helper (Th) lymphocytes play a key role in the pathogenesis of autoimmune diseases. As a new subset of lymphocytes, Th9 is thought to be involved in a wide range of disorders including rheumatoid arthritis (RA). In this study, we evaluated frequency of Th9 and Th2 cells and its correlation with disease activity in patients with different stages of RA.
Methods: The frequency of circulating interleukin 9- and/or interleukin 4-producing CD3+CD8-T cells was determined among 41 patients with established RA, 14 patients with very early RA (VERA) and 23 healthy controls by flow cytometry analysis. Then, correlation of cell frequencies with disease activity score 28 (DAS-28) was assessed. Serum levels of interleukin 6 and anti-citrullinated peptide antibodies were measured by enzyme-linked immunosorbent assay.
Results: Frequency of Th9 cells was significantly higher in RA patients compared to healthy controls (P=0.009). Moreover, mean percentage of circulating Th9 cells in patients with inactive VERA was significantly higher than that in those with active disease (P=0.046). In addition, mean percentage of Th9 cells had a negative correlation with the DAS-28 (r=-0.568, P<0.05). There was no significant correlation between the mean serum level of interleukin 6 and percentage of Th2 and Th9 cells (P>0.05).
Conclusion: Our results suggest that Th9 cells may have a potential role in RA initiation. Thus, targeting Th9 cells could be a promising strategy for advanced RA therapies.
Keywords: Rheumatoid arthritis, Th2 cells, Th9 cells.
Mohtaram Yazdanian, Mahtab Moazami, Mohammad Shabani, Sadegh Cheragh Birjandi,
Volume 13, Issue 6 (Nov-Dec 2019)
Abstract
ABSTRACT
Background and Objectives: Cerebral ischemia causes irreversible structural and functional damage in certain areas of the brain, especially in the hippocampus. The aim of this study was to examine effects of exercise preconditioning on neuronal cell death and expression of neurotrophin-4 (NT-4) and tropomyosin receptor kinase B (TrkB) in the hippocampal CA1 region following transient global cerebral ischemia/reperfusion in rat.
Methods: Twenty-one male Wistar rats (weighing 250-300 g) were randomly divided into three groups (control+healthy, control+ischemia and exercise+ischemia). The rats in the exercise group ran on a treadmill five sessions a week for eight weeks. Ischemia was induced by occlusion of both common carotid arteries for 45 minutes. Cresyl violet staining was performed to assess cell death, and real-time PCR was carried out to evaluate expression of NT-4 and TrkB.
Results: Cerebral ischemia was associated with significant neuronal death in the hippocampal CA1 region (P<0.05). Exercise significantly decreased the ischemia-induced cell death (P<0.05). NT-4 expression was significantly lower in the control+ischemia group and in the exercise+ischemia group compared to the control+healthy group (P<0.05), but there was no significant difference between the control+ischemia group and the exercise+ischemia group in terms of NT-4 expression (P˃0.05). Moreover, TrkB expression did not differ significantly between the groups (P˃0.05).
Conclusion: When used as a preconditioning stimulant before the induction of cerebral ischemia, exercise could have neuroprotective effects against cerebral ischemia-induced cell death, but it has no significant effect on NT-4 and TrkB expression.
Keywords: Exercise Preconditioning, Ischemia/Reperfusion, NT-4, TrkB, Cell death.
ABSTRACT
Background and Objectives: Cerebral ischemia causes irreversible structural and functional damage in certain areas of the brain, especially in the hippocampus. The aim of this study was to examine effects of exercise preconditioning on neuronal cell death and expression of neurotrophin-4 (NT-4) and tropomyosin receptor kinase B (TrkB) in the hippocampal CA1 region following transient global cerebral ischemia/reperfusion in rat.
Methods: Twenty-one male Wistar rats (weighing 250-300 g) were randomly divided into three groups (control+healthy, control+ischemia and exercise+ischemia). The rats in the exercise group ran on a treadmill five sessions a week for eight weeks. Ischemia was induced by occlusion of both common carotid arteries for 45 minutes. Cresyl violet staining was performed to assess cell death, and real-time PCR was carried out to evaluate expression of NT-4 and TrkB.
Results: Cerebral ischemia was associated with significant neuronal death in the hippocampal CA1 region (P<0.05). Exercise significantly decreased the ischemia-induced cell death (P<0.05). NT-4 expression was significantly lower in the control+ischemia group and in the exercise+ischemia group compared to the control+healthy group (P<0.05), but there was no significant difference between the control+ischemia group and the exercise+ischemia group in terms of NT-4 expression (P˃0.05). Moreover, TrkB expression did not differ significantly between the groups (P˃0.05).
Conclusion: When used as a preconditioning stimulant before the induction of cerebral ischemia, exercise could have neuroprotective effects against cerebral ischemia-induced cell death, but it has no significant effect on NT-4 and TrkB expression.
Keywords: Exercise Preconditioning, Ischemia/Reperfusion, NT-4, TrkB, Cell death.
Mohtaram Yazdanian, Mahtab Moazzami, Mohammad Shabani, Sadegh Cheragh Birjandi,
Volume 14, Issue 5 (Sep-Oct 2020)
Abstract
Background & Objective: Cerebral ischemia causes irreversible structural and functional damages in certain areas of the brain, especially the hippocampus. Evidence indicates that physical exercise may reduce the damages caused by cerebral ischemia. The purpose of this study was to examine the effect of 8-week exercise preconditioning on the expression of NT-3 and TrkC genes in the CA1 region of the hippocampus after the cerebral ischemic-reperfusion in male rats.
Methods: Twenty-one male Wistar rats weighing 250-300 gr were randomly selected and divided into three groups (healthy control, control + ischemia and exercise + ischemia). Rats in the exercise group ran on a treadmill 5 days per week for 8 weeks. Ischemia by occlusion of both common carotid arteries (CCA) was created for 45 minutes. In order to evaluate the gene expression, Real time PCR technique was used.
Findings: NT-3 gene expression was significantly different between exercise + ischemia with control + ischemia groups and control + ischemia with healthy control groups (P <0.05), and TrkC gene expression was significantly different between exercise + ischemia with healthy control groups and control + ischemia with healthy control groups (P <0.05).
Conclusion: The results of this study demonstrated that exercise before the induction of ischemic stroke increased the NT-3 gene expression but did not influence the TrKC gene expression.
Zinat Yazdani, Iman Baluchi, Behjat Kalantary Khandany, Gholamhosein Hassanshahi,
Volume 15, Issue 2 (Mar-Apr 2021)
Abstract
Background and objectives: Acute myeloid leukemia (AML) is a heterogeneous malignancy caused by various pathological mechanisms. Chemokines are involved in the initiation, progression, migration, survival, treatment and complications of AML. CXCL1 has an indirect effect on the progression of cancer and CXCL10 produced by leukemia cells attracts natural killer cells toward tumor sites to eradicate cancer cells. The present study investigated effects of chemotherapy on serum levels of CXCL1 and CXCL10 in patients with AML.
Methods: Throughout this case-control study, blood samples were collected from AML patients with M4/M5 subtype (n=25) before and after the first stage of a chemotherapy regimen (7+3). Serum levels of the chemokines were determined using commercial ELISA kits. Data were analyzed using two-sample and paired T-test in SPSS 22 software.
Results: The level of CXCL10 was high in patients but decreased following chemotherapy. After the chemotherapy the patients attained partial remission. However, the level of CXCL1 did not change in the patients.
Conclusion: Although chemotherapy could decrease CXCL10 levels and induce partial remission, CXCL1 levels does not change in AML patients with M4/M5 subtype. Based on the results, the employment of CXCL1 and CXCL10 inhibitors in the chemotherapy regimen could prevent relapse in the later stages or even reduce the duration of treatment.
Bahar Yazdani, Hussein Anani, Iman Baluchi, Behjat Kalantary Khandany, Gholamhossein Hassanshahi,
Volume 15, Issue 4 (Jul-Aug 2021)
Abstract
Background and objectives: Acute myeloid leukemia (AML) is a malignancy that involves the bone marrow and peripheral blood. Some chemokines play a role in the progression, migration and tumor initiation and are therefore associated with poor prognosis. CCL2 promotes tumor growth and is associated with poor prognosis in AML patients. We investigated effects of chemotherapy on serum level of CCL2 in AML patients.
Methods: Throughout this case-control study, blood samples were collected from 25 healthy individuals and 25 AML (M4 and M5) patients before and after the first stage of the current chemotherapy regimen (7+3). Serum level of CCL2 was measured using commercial ELISA kits. Data were analyzed in SPSS 22 using the two-sample t-test and paired t-test.
Results: Before chemotherapy, serum level of CCL2 was significantly higher in the patients than in the healthy controls. Following chemotherapy, the serum level of CCL2 reduced significantly to a level comparable to that of the healthy controls.
Conclusion: The current chemotherapy (7+3) can effectively inhibit CCL2 in AML patients.
