S Shojaee, F Hosseine, N Fakhr-E-Rad, B Bijaree, M Sekhavati, M Fereidouni,
Volume 7, Issue 2 (summer[PERSIAN] 2013)
Abstract
Abstract
Background and Objective: Having knowledge about potential risks and the usage of safety equipment in laboratories can decrease the risk of occupational exposure. The aim of this study was to evaluate the predisposing factors for occupational exposure and to assess the usage of safety equipment among personnel of medical diagnostic laboratories in Birjand.
Material and Methods: In this descriptive analytic study, all staff of laboratories was assessed by using a questionnaire including demographic data, type of accidental exposures and the use of protective equipment. Using SPSS software, we analyzed the data.
Results: Of 110, 84 (76%) have at least one accidental exposure to patients’ specimens in that 55% of accidents are related to sample preparation step. In 82% of contacts, carelessness is the underlying cause of exposure. Gown, glove, fume hood and mouth mask are routinely used by 97%, 48%, 34%, 1% of personnel, respectively. Nearly all of (97%) personnel were vaccinated against hepatitis B and 78% of them have performed routine blood test for detecting any infections.
Conclusion: The results of current study show that accidental exposures to patients’ samples are common among personnel of medical diagnostic laboratories. The level of preventive education and the rate of safety equipment usage are low therefore, we recommend planning of some training sessions to persuade the personnel for using safety equipment.
Keywords: Medical laboratory, Occupational Exposure, Specimens, Vaccination
M Eramabadi, K Tadayon, N Mosavari, R Keshavarz, R Banihashemi, R Ghaderi, M Sekhavati, M Ahmadi, P Eramabadi, E Khodaverdi Daryan,
Volume 7, Issue 5 (supplement Issue( Bacteriology)[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: A high level of homogeneity observed within all bacteria in the Mycobacterium tuberculosis complex makes a property that seriously challenges traditional biochemical-based identification methods of these pathogens in the laboratory. The work presented here was conducted to characterize Mycobacterium tuberculosis complex isolates in Golestan, Northern Iran.
Material and Methods: Between 2008 and 2010, 42 mycobacterial isolates were collected from clinical tuberculosis-suspected patients in Golestan province. The isolates were sub-cultured on fresh Mycobacterium-specific culture media including glycerinated and pyruvated Lowenstein-Jensen slopes. The isolates were subsequently subjected to a PCR-based identification scheme coined Huard-Warren method. This strategy consisted of three individual algorithms namely, 16SrRNA RV typing (Rv0577, Rv3877.8, Rv1970, Rv3120, Rv1510 and IS1561) and RD typing (RD1, RD 4, RD9 and RD12).
Results: All isolates were proved to be M. tuberculosis. Furthermore, none of the patients were being infected with any other member of the M. tuberculosis complex or simultaneously co-infected with two mycobacteria. This fundamental observation was independently obtained by specific culture media, RV typing and also RD typing.
Conclusion: Considering the fact that cattle and sheep farming play an important role in the economy of the region, absence of Mycobacterium bovis in the studied isolates can be unexpected to some extent. Huard-Warren which is a simple and cost-effective identification method can be used in both reference and regional laboratory for differential diagnosis of tuberculosis.
Keywords: Mycobacterium Tuberculosis Complex, Huard-WarrenMethod, 16SrRNA, Golestan Province, RD Typing, RV Typing
