Showing 7 results for Jamshidi
Hr Pordeli, Sj Hashemi Hazaveh, M Jamshidian, M Bayat,
Volume 7, Issue 2 (summer[PERSIAN] 2013)
Abstract
Abstract Background and objective: Soil bacteria, particularly Bacillus genus have the potential of producing a range of bioactive substances with antimicrobial and antifungal properties. They have the ability to produce hundreds of active and effective biologic compound against microorganisms. Therefore, it seems to be a proper candidate in the biocontrol of fungal pathogenesis. Material & Methods: In this study, soil samples were collected from different parts of Gorgan in order to isolate Bacillus and to determine their antifungal activity against T.mentagrophytes. The Isolates that had the highest antifungal effects were analyzed by PCR and 16s rRNA sequencing. Results: of 54 strains, 14 have antifungal activity. The Isolates, S4 and S12, identified as B.cereus and B.thuringiensis respectively show the highest antidermatofit effect. These isolates based on 16s rRNA sequence analysis show 97% homology with Bacillus cereusstrain KU4 and Bacillus thuringiensisstrain ucsc27. Conclusion: According to the results, it seems that the soil Bacilli have biocontrol potential against dermatophytic agents such as T.mentagrophytes. Keywords: Antifungal effects, Bacillus, Rhizospheric soil, T.mentagrophytes
A Ebrahimzadeh, S Mohammadi, T Davoodi, Ar Salimi Khorashad, A Jamshidi,
Volume 7, Issue 3 (Autumn 2013)
Abstract
Abstract
Background and Objective: Toxoplasmosis is one of the most prevalent parasitic infections worldwide. Contamination of pregnant women with toxoplasmosis may cause fetal death, preterm delivery and congenital toxoplasmosis. Due to importance of congenital Toxoplasmosis and the need of further study, this research was accomplished in Zahedan.
Material and Methods: The serum samples (N= 221) were collected from pregnant women referring to reference laboratory of Zahedan in 2011. The IgG and IgM antibody levels against toxoplasmosis were investigated using ELISA method.
Results: Out of all samples, 30.8% are IgG positive and 1.4% are both IgG and IgM positive. There is no significant difference between positive and negative groups using Chi-square tests.
Conclusion: The main part of pregnant women in Zahedan (69.2%) is serologically negative against toxoplasmosis therefore, hygiene education to eliminate risk factors especially during pregnancy period seems to be imperative.
Keywords: ELISA Antibody Pregnancy Toxoplasma Zahedan
A Jamshidi, M Mousavi Ghahfarrokhi, A Gharaei, A Ebrahimzadeh, M Jaffari Modrek, A Ansari Moghadam, S Mohammadi,
Volume 8, Issue 1 (spring[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: The Plasmodium falciparum EBA-175, via Sialic acid dependent glycophorin A, binds to red blood cells and thus plays a critical role in cell invasion. Some part of second allele in its gene encoding in FCR-3 (Section F) and CAMP (Section C) can be found. This study aimed to determine the prevalence of Plasmodium falciparum EBA-175KD alleles in southeastern Iran.
Material and Methods: In this cross-sectional study, using polymerase chain reaction Nest (Nested-PCR) with specific primers was used for the two parts of the EBA-175 gene to be proliferated. Ninety–four microscopic positive blood samples from individuals infected by Plasmodium falciparum were obtained from four different locations in southeastern Iran.
Results: Of 94 positive samples, 88 were antigen EBA-175KD. Genotype CAMP (714 bp) and FCR-3 (to 795 bp), respectively, in 31 (32.97 %) and 49 (52.12 %) were found. Eight samples have both FCR-3 and CAMP.
Conclusion: Both of EBA-175KD dimorphic genes were found. The frequency of FCR-3 allele was higher in the South East of Iran. Thus, this pattern can be considered in making Plasmodium falciparum vaccines for this area.
Key words: Plasmodium Falciparum Erythrocyte Binding Antigen-175 South-East of Iran
Ebrahimzad, Mousavi Moh, Jamshidi Ali, Mohammadzadeh Rostami F (msc),
Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)
Abstract
Background and Objective: Oral candidiasis is a common opportunistic infection in diabetic patients. Candida albicans is colonized on different oral surfaces such as tongue, palate, dental caries and plaques. This study aimed to determine the prevalence of Candida albicans in the mouth of diabetic patient.
Material and Methods: This cross - sectional study was conducted on 120 diabetic patients. After completing the questionnaire, the saliva samples were obtained and cultured on sabouraud agar medium containing 0.005% chloramphenicol. For final diagnosis, Germ tube test and Sugar absorption test (with API 120 kit) were used.
Results: Of 120 patients, 61 (50.8%) were positive for Candida. Candida infection rate was relatively higher in older patients and in women. The rate was significant in the patients using dentures (P< 0.001) and the ones keeping on smoking (P <0.038). The infection in the patients with type II diabetes was higher than the cases with Type I.
Conclusion: Given the high prevalence of Candidiasis and Candida colonization in the mouth of diabetic patients, it is a necessity to identify and treat these infections in order to prevent further complications.
Keywords: Candidiasis, Diabetes Mellitus, Fungal Infections
Behzad Ghasemi, Mohsen Najimi, Hamid Beyzaei, Abbas Jamshidian,
Volume 9, Issue 4 (sep,Oct 2015 2015)
Abstract
Abstract
Background and Objectives: Antibiotic resistance in bacteria has actuated researchers toward evaluating many new antibacterial compounds of which are the thiazoles. In this research the inhibitory effects of novel thiazole derivatives were unraveled on Staphylococcus aureus, Streptococcus agalactiae, Pseudomonas aeruginosa and Klebsiella pneumoniae and oxothiazole liver toxicity effects were assessed on mice.
Methods: The antibacterial effect of thiazole derivatives was evaluated by measuring the halo zone with disk diffusion method and dilution procedure in microplate in order to discriminate the minimum inhibitory concentration (MIC) and the liver toxicity of oxothiazole, also, was discerned by injecting 160 mg/kg, 265 mg/ kg and 350 mg/kg doses to mice as well as scrutinizing the liver histopathology.
Results: Derivatives utilized in experiment had no inhibitory effect on Pseudomonas aeruginosa and Klebsiella pneumoniae, though their inhibitory effect was observed on Staphylococcus aureus and Streptococcus agalactiae. For Staphylococcus aureus and Streptococcus agalactiae the diameters of growth inhibition zone were 8.9-22.3 mm and 16.1-25.6 mm, respectively and MIC of 50-200 and 25-100 µg/ml by order. Additionally, by increasing the injection dose of oxothiazole with 160 mg/ml, 265 mg/ml and 350 mg/ml doses, the hepatitis lesions and liver necrosis were observed in experimental mice.
Discussion: The thiazole derivatives possessed more inhibitory trace on gram positive bacteria than gram negative ones. Furthermore, the likely presence of oxygen link to thiazole ring in tested compounds results in the enhancement of inhibitory potency of these substances. Besides, our results suggest that high doses of oxothiazole cause severe liver damage and rapid death less than 24 hours.
Keywords: Thiazole derivatives, Antibacterial effects, Oxothiazole, Liver toxicity.
Mohammad Niakan, Noushin Jalayer Naderi , Hadise Jamshidian, Fateme Jafariazad,
Volume 11, Issue 4 (Jul-Aug 2017)
Abstract
ABSTRACT
Background and Objective: Anaerobic bacteria are the main cause of periodontitis. It has been shown that green tea and black tea have antibacterial effect. The aim of this study was to determine he antibacterial effect of Iranian green tea and black tea against
Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and
Prevotella intermedia.
Methods: Aqueous and methanolic extracts of Iranian green tea and black tea at concentrations ranging from 10 to 500 mg/ml were tested against standard strains of
A. actinomycetemcomitans (ATCC 33384),
P. gingivalis (ATCC 33227) and
P. intermedia (ATCC 25671) using agar disk diffusion, broth microdilution and determination of minimum inhibitory concentration.
Results: P. gingivalis, A. actinomycetemcomitans and
P. intermedia were sensitive to the methanolic extract of Iranian green tea at concentrations of 100-500 mg/ml, 10-500 mg/ml and 50-500mg/ml, respectively.
P. gingivalis, A. actinomycetemcomitans and
P. intermedia were sensitive to the methanolic extract of Iranian black tea at concentrations of 200-500 mg/ml, 20-500 mg/ml and 200-500 mg/ml, respectively.
In addition, P. gingivalis, A. actinomycetemcomitans and
P. intermedia were sensitive to the aquatic extract of Iranian green tea at concentrations of 200-500mg/ml, 100-500 mg/ml and 200-500 mg/ml
, respectively.
Conclusion: The aquatic and alcoholic extracts of Iranian green tea and black tea have antibacterial activity against
A. actinomycetemcomitans,
P. intermedia and
P. gingivalis. Therefore, incorporation of Iranian black tea as an effective native herb could be beneficial for prevention of oral cavity diseases.
Keywords: Tea, Green Tea, Antibacterial Agents, Anaerobic Bacteria.
Seyed Ahmad Sajjadi, Zahra Moosavi, Farhad Niknejad, Abdollah Jamshidi,
Volume 17, Issue 4 (Jul-Aug 2023)
Abstract
Seyed ahmad Sajjadi1 
,
Zahra Moosavi2 ,
Farhad Niknejad3 ,
Abdollah Jamshidi 4
Background: Aflatoxin B1 (AFB1) is one of the most important mycotoxins that contaminate food worldwide. Long-term consumption of foods contaminated with AFB1 endangers human health. Detoxification of AFB1 from food improves community health. A Specific approach to aflatoxin reduction is the use of probiotics. Kefir drink is a strong probiotic. The purpose of this study was to investigate the protective effect of kefir drink on AFB1-induced hepatic injury in adult male rats
Methods: In this experimental study, 24 adult rats weighing between 150 and 200 g were used. The rats were randomly divided into 4 groups: 1) control, 2) AFB1 (50 μg/kg body weight), 3) kefir drink (10 mL/kg body weight), and 4) AFB1 + kefir drink. Aflatoxin and kefir drink received through oral gavage. At the end of the experiment (8 weeks), blood and liver samples were collected for different assays. Liver function tests and histopathological examinations were performed. Data were analyzed using 1-way analysis of variance (ANOVA) and at a significance level of <0.05.
Results: Aflatoxin B1 significantly increased serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin (T.Bili), as well as decreased total protein (T.P) content, compared to the control group (P < 0.05). Aflatoxin B1 induced histological changes in the liver. The results obtained from the groups treated with kefir drink with and without AFB1 were not significantly different from the control group. Histopathological changes were not found in groups treated with kefir drink with and without AFB1.
Conclusion: The consumption of kefir drink reduced AFB1-induced disruptions in rats’ livers.
