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Volume 13, Issue 5 (Sep-Oct 2019)
mljgoums 2019, 13(5): 32-37 |
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Keyghobadi F, Bahramifar N, Gharekhani E, Kia M. Detection of Trace Amounts of Selenium Ions in Water and Blood Samples by Solid Phase Extraction Using Thiolated Nanosilica. mljgoums 2019; 13 (5) :32-37
URL: http://mlj.goums.ac.ir/article-1-1131-en.html
URL: http://mlj.goums.ac.ir/article-1-1131-en.html
1- (MSc) Department of Analytical Chemistry, Golestan University of Medical Sciences, Gorgan, Iran , fereshteh_keyghobadi@yahoo.com
2- (PhD) Department of Analytical Chemistry, Tarbiat Modares University, Noor, Iran
3- (MSc) Department of Toxicology, Golestan University of Medical Sciences, Gorgan, Iran
4- (MSc) Department of Food Sciences, Golestan University of Medical Sciences, Gorgan, Iran
2- (PhD) Department of Analytical Chemistry, Tarbiat Modares University, Noor, Iran
3- (MSc) Department of Toxicology, Golestan University of Medical Sciences, Gorgan, Iran
4- (MSc) Department of Food Sciences, Golestan University of Medical Sciences, Gorgan, Iran
Abstract: (3825 Views)
ABSTRACT
Background and Objectives: In this study, nanosilica modified with HS-SiO2 thiol groups was utilized as adsorbent for solid phase extraction, as a fast and reliable method of preconcentration and separation of very small quantities of selenium ions from water and blood samples.
Methods: The samples included four natural water samples and one biological sample (blood serum) prepared in volumes of 25, 100, 200, 300, 400 and 500 ml. The samples were analyzed by solid phase microextraction, using thiolated-nanosilica (as adsorbent), ultraviolet-visible spectrophotometry and atomic absorption spectroscopy.
Results: Optimized conditions for preconcentration of a 25 ml 0.2 mg/l selenium solution were pH 5, 40 mg of adsorbent, sample-adsorbent mixing time of 15 minutes and 5 ml of 2N sulfuric acid as detergent. The volume limit and concentration factor were 400 and 80, respectively. Limit of detection and relative standard deviation of the method were 0.46 μg/l and 0.9%, respectively.
Conclusion: This study is the first to successfully utilize thiolated nanosilica for measuring low selenium levels. Thiolation of the absorbent increases selenium adsorption by thiolated-silica compared to SiO2.
Keywords: Solid phase extraction, Selenium, Preconcentration, Nano, UV-visible spectrophotometry.
Background and Objectives: In this study, nanosilica modified with HS-SiO2 thiol groups was utilized as adsorbent for solid phase extraction, as a fast and reliable method of preconcentration and separation of very small quantities of selenium ions from water and blood samples.
Methods: The samples included four natural water samples and one biological sample (blood serum) prepared in volumes of 25, 100, 200, 300, 400 and 500 ml. The samples were analyzed by solid phase microextraction, using thiolated-nanosilica (as adsorbent), ultraviolet-visible spectrophotometry and atomic absorption spectroscopy.
Results: Optimized conditions for preconcentration of a 25 ml 0.2 mg/l selenium solution were pH 5, 40 mg of adsorbent, sample-adsorbent mixing time of 15 minutes and 5 ml of 2N sulfuric acid as detergent. The volume limit and concentration factor were 400 and 80, respectively. Limit of detection and relative standard deviation of the method were 0.46 μg/l and 0.9%, respectively.
Conclusion: This study is the first to successfully utilize thiolated nanosilica for measuring low selenium levels. Thiolation of the absorbent increases selenium adsorption by thiolated-silica compared to SiO2.
Keywords: Solid phase extraction, Selenium, Preconcentration, Nano, UV-visible spectrophotometry.
Research Article: Original Paper |
Subject:
Biochemistry
Received: 2018/10/31 | Accepted: 2019/01/13 | Published: 2019/09/2 | ePublished: 2019/09/2
Received: 2018/10/31 | Accepted: 2019/01/13 | Published: 2019/09/2 | ePublished: 2019/09/2
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