Jorjani Biomedicine Journal

en Inhibitory effects of Echinophora platyloba L. ethanol extract on the development of morphine dependence: An in vivo study using naloxone-precipitated withdrawal symptoms in male mice علوم پایه پزشکی Basic Medical Sciences تحقیقی Original article Background: Promising results were obtained by using medicinal plants for the treatment and prevention of opioid withdrawal syndrome. A native Iranian plant, Echinophora platyloba, has shown analgesic effects that may be mediated through opioid receptors. Thus, the present research evaluated the effects of the ethanolic extract of E. platyloba on the acquisition of morphine dependence in mice. Methods: Thirty-two male mice were randomly allocated into four groups of eight. Morphine dependence was induced in the mice by subcutaneous administration of morphine (50, 50, and 75 mg/kg)×3 days, plus a single dose of morphine (50 mg/kg) on the fourth day. Withdrawal syndrome was precipitated by intraperitoneal injection of naloxone (4 mg/kg) 2 hours after the last dose of morphine. On days of dependence induction and before each morphine dose, the mice received intraperitoneal injections of saline (10 ml/kg) or plant extract (25, 50, and 75 mg/kg). After the injection of naloxone, the symptoms of withdrawal syndrome in each animal were monitored for 30 minutes. Results: Administering morphine with this method induced morphine dependence in mice that were treated with saline before morphine. However, the mice that received the plant extract showed a significant decrease in the withdrawal symptoms including the number of jumping (P<0.01), rearing (P<0.05), grooming (P<0.05), and diarrhea (P<0.01) compared with the saline-treated mice. Conclusion: Administration of the hydro-ethanolic extract of E. platyloba before morphine may inhibit the induction of morphine dependence. Therefore, the plant extract may be considered a therapeutic agent for the prevention of morphine dependence in morphine users. Morphine dependence,Plant extracts,Naloxone,Substance withdrawal syndrome,Mice 9 13 http://goums.ac.ir/jorjanijournal/browse.php?a_code=A-10-956-1&slc_lang=en&sid=1 Amir Abbas Barzegari barzegaridoctora@gmail.com 100319475328460010838 0000000320734696 Yes Department of Biology, Faculty of Basic Science, University of Maragheh, Maragheh, Iran Ahmad Aghaee aghaee2001@yahoo.com 100319475328460010839 0000000324779330 No Department of Biology, Faculty of Basic Science, University of Maragheh, Maragheh, Iran Kamran Shahabi shahabikamran@gmail.com 100319475328460010840 0000000333314367 No Department of Biology, Faculty of Basic Science, University of Maragheh, Maragheh, Iran