Majid Amini Khorasgani , Parisa Mohammady Nejad, Mohammad Mehdi Moghani Bashi,
Volume 6, Issue 4 (12-2018)
Abstract
Background and objectives: One of the latest studies in the genetics field is the evaluation of role of micro-RNAs as a biomarker for diagnosis of multiple sclerosis (MS), which is a demyelinating disease of the central nervous system (CNS) and emerges in the form of numerous small and large plaques in white matter in the brain and spinal cord. This disease could be associated with several complications, including reduced vision, spasticity and imbalance, and impaired sphincter control. MiR-202-3p is an intronic miRNA located in the ADA (Adenosine Deaminase) gene, which is the main enzyme involved in the pathway for the conversion of adenosine into inosine. Moreover, ADA regulates the inflammatory response and protection of tissue from damage as a strong complementary mechanism. This study aimed to evaluate the expression level of miR-202-3p in individuals diagnosed with relapsing-remitting multiple sclerosis (RRMS) and healthy individuals in Isfahan, Iran.
Methods: This analytical-observatory study was performed on 49 RRMS patients and 52 healthy individuals with no history of autoimmune and inflammatory diseases. Total RNA was extracted from blood lymphocytes by Ficoll and Trizol. Afterwards, cDNA was formed using a special miRNA cDNA kit, followed by the application of Real-time RT PCR to measure the expression of miR-202-3p in healthy individuals and patients.
Results: According to the results, the miR-202-3p expression was higher in patients, compared to healthy individuals (P=0.006). In addition, the sensitivity and diagnostic value of this miRNA in receiver operating characteristic (ROC) curve analysis were equal to AUC=0.80 (area under the curve).
Conclusion: In line with other studies, our findings demonstrated that miR-2023p can be used as a biomarker in the diagnosis of MS. In addition, it seems that miR-202-3p acts as an immunosuppressant by inhibiting ADA gene, which regulates various processes related to inflammatory response and maintenance of tissue from damage.
Ali Esmaeili , Abdolhamid Habibi , Mohammad Rami , Mehrzad Shabani ,
Volume 13, Issue 1 (9-2025)
Abstract
Background: Performing exercise training with various protocols, especially aquatic exercises, can be effective against the harmful effects of Multiple Sclerosis. Therefore, the present study aimed to investigate the effect of six weeks of swimming training on the Caspase-1, TGF-β1, and IFN-γ protein content in the hippocampal tissue of rats with Multiple Sclerosis.
Methods: Twenty-one Wistar rats were divided into three equal groups: (1) Healthy control, (2) Multiple Sclerosis control, and (3) Multiple Sclerosis swimming. After two weeks of adaptation to the laboratory environment, the Multiple Sclerosis groups were induced by adding cuprizone to their diet. Six weeks of swimming training were then performed. Forty-eight hours after the last session, hippocampal tissue was isolated to examine Caspase-1, TGF-β1, and IFN-γ protein content. To analyze the data, one-way analysis of variance was used with a significance level of 0.05.
Results: Findings showed that the induction of Multiple Sclerosis in rats caused a significant increase in TGF-β1 and Caspase-1 protein content (P-Value=0.001) and a significant decrease in IFN-γ (P-Value =0.001). After six weeks of swimming, there was a significant decrease in Caspase-1 (P-Value =0.001) and a significant increase in IFN-γ (P-Value =0.001) protein content; however, there was no significant decrease in TGF-β1 protein content (P-Value =0.1).
Conclusion: Based on the findings of this study, it can be concluded that swimming, as a non-pharmacological intervention, has a protective effect on nerves by reducing factors related to inflammation and cell death, which may have beneficial effects on memory information processing in Multiple Sclerosis disease.
