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Showing 4 results for saeid

Sanaz Baghbanrahimi, Hoorieh Soleimanjahi, Alireza Mohebbi, Mir Saeid Ebrahimzadeh, Leyla Alizade, Amir Ghaemi,
Volume 5, Issue 1 (5-2017)
Abstract

Background & Objective: Human papillomavirus (HPV) oncoproteins, including E6 and E7 are constitutively expressed in cervical cancer cells. These proteins are ideal targets to be used for developing therapeutic vaccines against existing HPV-associated carcinomas. The aim of this study was to measure the proliferation response rate of splenic lymphocytes derived from E7-HPV16 encoding plasmid injection on the tumor mouse model of papillomavirus.
Method: C57BL/6 mice were inoculated subcutaneous with 5× 10⁵ TC-1 cells in three times with two weeks intervals and then immunized with HPV-16 E7 DNA vaccine. The proliferation response of splenic cells was measured by MTT assay. IL12 cytokine was measured by ELISA assay and the mass of tumor was calculated with caliper for six weeks.
Results: Following the application of DNA vaccines containing E7 therapeutic gene, the proliferative response of splenic cells was provoked significanltly higher than the stimulation in control group (P<0.05). Moreover, the secretion of IL12 was significantly increased in vaccinated mice tumor tissue (P<0.05). The growth of tumor in vaccinated group was markedly decreased in comparison to PBS and pcDNA3 groups (P<0.05).
Conclusion: Our findings revealed that the application of DNA vaccine containing E7 gene in a tumor mouse model may induce anti-tumor cellular immune responses.

Zahra Bahrololoomi, Fateme Mehravar, Niloofar Halvani, Hamed Saeid,
Volume 10, Issue 2 (5-2022)
Abstract

Background and objectives: Microleakage at the interface of tooth structure and fissure sealant plays a crucial role in failure of the treatment. This in vitro study aimed to determine the microleakage of self-adhesive flowable composite resins as pit and fissure sealants.
Material and Methods: 60 healthy extracted premolar teeth were randomly divided into the 4 groups (n=15), group 1) clinpro sealants (control), group 2) flowable composite resin with bonding agent (total-etch), group 3) flowable composite resin with self-etch bonding agent, and group 4) self-adhesive composite resin. After thermocycling the specimens were immersed in 2% methylene blue for 48 hours and then sectioned in the buccolingual direction. The microleakage was assessed by dye penetration using a stereomicroscope at 15X magnification. Data analysis was performed using SPSS version 18.0 as well as Mann-Whitney and Kruskal-Wallis tests.
Results: There was a statistically significant difference in microleakage score between the groups (P-value = 0.006). The lowest mean of microleakage level was observed in the flowable composite group with total-etch bonding (group 2) 1.26±0.96, followed by the Clinpro sealant group (group 1) 1.62±1.20, and then the self-adhesive composite group (group 4) 1.85±1.00.
Conclusion: Based on the results of this study, the microleakage of self-adhesive composite resin revealed no significant difference with conventional fissure sealant; however, the microleakage of these composite resin was higher than flowable composite resin with total-etch bonding agent.

Mahmoud Mohammadi, Leila Kohan, Mohsen Saeidi, Marie Saghaeian-Jazi, Saeed Mohammadi,
Volume 10, Issue 2 (5-2022)
Abstract

Fibrosis is a common and mostly progressive pathological outcome in various chronic inflammatory disorders. Dermal (skin) fibrosis, which is associated with intense skin lesions, is a result of an uncontrolled healing process in the dermis, particularly disproportionate fibroblast proliferation and extracellular matrix (ECM) production. Animal models are substantial tools in biomedical investigations and have been considerably employed to evaluate miscellaneous features of diseases that cannot be demonstrated otherwise in humans. To date, various skin fibrosis models have been generated, including the transgene and/or genetic models and chemical and drug-induced models. However, genetic models are sophisticated and need access to convoluted methods. Accordingly, the introduction of affordable and easy to generate fibrosis models in the skin is crucial. Here, we aimed to introduce the chemical/drug-induced skin fibrosis animal models to provide an updated list of available approaches.
 

Fatemeh Mohajer, Saeid Khanzadi, Mohammad Hashemi, Mohammad Azizzadeh,
Volume 10, Issue 3 (10-2022)
Abstract

Background and Objectives: Nowadays, to avoid the use of synthetic preservatives, which do more harm than good, numerous studies are currently focused on using natural ingredients to enhance food product quality and shelf life. Since no study has been conducted on combining coatings with electrolyzed water containing natural antimicrobial compounds, the present study has innovation and priority. In the present study, the effect of chitosan coating prepared through Neutral Electrolyzed Water (NEW) on inhibiting the growth of Escherichia coli O157:H7 inoculated in rainbow trout fillet over 12 days at 4 ˚C was examined.
Material and Methods: Fish samples were allocated into six groups following inoculation with E. coli O157:H7 (final concentration: ~ 105 CFU/g). Treatments included control (CON), distilled water (DW), neutral electrolyzed water (NEW), chitosan 2% (CH), chitosan coating prepared by neutral electrolyzed water (CH/NEW), and neutral electrolyzed water followed by chitosan (NEW+CH). Treatments were kept at low temperature (refrigerator) and counting bacteria was done on 0, 1st, 3rd, 6th, 9th, and 12th days. Data analyses were done through repeated measure ANVOA and Bonferroni post hoc tests.
Results: As indicated by the findings, the reduction rate of EW, CH, CH+WE, and CH/EW samples were 1.04, 1.45, 2.01, and 2.02 log CFU/g compared with the CON, respectively. The highest reduction rate was observed in the CH/NEW sample, due to the antimicrobial activity of chitosan and neutral electrolyzed water.
Conclusion: Chitosan coating can be combined with NEW in fish to increase the safety against pathogenic bacteria and E. coli O157:H7. Therefore, it can suggest the use of these natural antimicrobial compounds in the food industry.




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