Volume 11, Issue 2 (10-2023)
Jorjani Biomed J 2023, 11(2): 24-27 |
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Jamali A, Zare Ebrahimabad M, Zhand S, khosravi A. Monocyte chemoattractant protein-1 (MCP1) -2581 A/G and interferon gamma (IFNγ) +874 T/A polymorphisms in Iranian population with pulmonary tuberculosis. Jorjani Biomed J 2023; 11 (2) :24-27
URL: http://goums.ac.ir/jorjanijournal/article-1-986-en.html
URL: http://goums.ac.ir/jorjanijournal/article-1-986-en.html
1- Laboratory science research center, Golestan University of Medical Sciences, Gorgan, Iran
2- Department of Biochemistry, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
3- .Institute for Biomedical Materials and Devices, Faculty of Science, University of Technology Sydney, New South Wales 2007, Australia
4- Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran , ayyoobfarsian@yahoo.com
2- Department of Biochemistry, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
3- .Institute for Biomedical Materials and Devices, Faculty of Science, University of Technology Sydney, New South Wales 2007, Australia
4- Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran , ayyoobfarsian@yahoo.com
Abstract: (964 Views)
Background: Genetic polymorphisms are predictors of the immune response and susceptibility to certain infectious diseases, including pulmonary tuberculosis (TB). We evaluated the association of monocyte chemoattractant protein-1 (MCP1) (-2581 A/G) and interferon-gamma (IFNγ) (+874 T/A) polymorphisms with susceptibility to pulmonary TB in an Iranian population.
Methods: A total of 124 patients with pulmonary tuberculosis and 244 healthy subjects (121 related normal controls and 123 unrelated subjects) were included. The MCP1 polymorphic region (-2518 A/G) was genotyped by PCR-RFLP, while ARMS-PCR was used to amplify and detect IFNγ (+874 T/A). SNPStats and SPSS v. 20 were used for the statistical analysis of the data.
Results: The comparison of MCP1 (-2518 A/G) alleles and genotypes in TB patients and healthy subjects showed no significant association in all the constructed heredity models. No association was observed between TB patients and normal subjects in all the constructed inheritance models for IFNγ (+874 T/A) alleles and genotypes.
Conclusion: Due to the lack of association between MCP1 (-2518 A/G) and IFNγ (874 T/A) polymorphisms and susceptibility to PT in our study and the conflicting results of some previous studies, further clinical and molecular research is needed to clarify the role of the studied polymorphisms in the pathogenesis of tuberculosis.
Methods: A total of 124 patients with pulmonary tuberculosis and 244 healthy subjects (121 related normal controls and 123 unrelated subjects) were included. The MCP1 polymorphic region (-2518 A/G) was genotyped by PCR-RFLP, while ARMS-PCR was used to amplify and detect IFNγ (+874 T/A). SNPStats and SPSS v. 20 were used for the statistical analysis of the data.
Results: The comparison of MCP1 (-2518 A/G) alleles and genotypes in TB patients and healthy subjects showed no significant association in all the constructed heredity models. No association was observed between TB patients and normal subjects in all the constructed inheritance models for IFNγ (+874 T/A) alleles and genotypes.
Conclusion: Due to the lack of association between MCP1 (-2518 A/G) and IFNγ (874 T/A) polymorphisms and susceptibility to PT in our study and the conflicting results of some previous studies, further clinical and molecular research is needed to clarify the role of the studied polymorphisms in the pathogenesis of tuberculosis.
Keywords: Monocyte chemoattractant protein-1, Interferon-gamma, Genetic Polymorphism, Pulmonary tuberculosis
Type of Article: Original article |
Subject:
Molecular Sciences
Received: 2023/09/9 | Accepted: 2023/10/8 | Published: 2023/12/30
Received: 2023/09/9 | Accepted: 2023/10/8 | Published: 2023/12/30
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